24 US AI Startups That Have Secured $100M or More in Funding in 2025

Last year marked a significant milestone for the AI industry in the U.S. and globally. In 2024, 49 startups successfully raised funding rounds of $100 million or more, with three companies securing multiple “mega-rounds” and seven companies raising rounds exceeding $1 billion.

As we delve into 2025, the trend appears to continue robustly. The first half of the year shows promising signs, with several billion-dollar rounds already completed, surpassing the Q1 performance of the previous year. Here’s a snapshot of the U.S. AI startups that have raised $100 million in 2025:

June

• Glean, an enterprise search startup, announced a $150 million Series F round on June 10, bringing its valuation to $7.25 billion. The round was led by Wellington Management with notable participation from Sequoia and Lightspeed.
• Anysphere, the AI research lab behind the coding tool Cursor, secured $900 million in a Series C round. This funding elevated its valuation to nearly $10 billion, led by Thrive Capital, with involvement from Andreessen Horowitz and Accel.

May

• Snorkel AI, an AI data labeling startup, raised $100 million in a Series D round on May 29, reaching a valuation of $1.3 billion. The round was led by Addition Ventures with backing from several others.
• LMArena, a benchmarking tool for AI models, raised $100 million seed round on May 21, valuing it at $600 million. Key investors included Andreessen Horowitz and UC Investments.
• TensorWave, based in Las Vegas, announced a $100 million Series A round on May 14, co-led by Magnetar Capital and AMD Ventures.

April

• SandboxAQ closed a $450 million Series E round on April 4, with a valuation of $5.7 billion. Investors included Nvidia and Google.
• Runway, specializing in AI models for media production, raised $308 million in Series D funding on April 3, achieving a valuation of $3 billion.

March

• OpenAI astoundingly raised $40 billion, valuing it at $300 billion on March 31. This monumental round was led by SoftBank.
• Other notable raises included Nexthop AI‘s $110 million Series A, Insilico Medicine‘s $110 million for drug discovery, and Celestial AI‘s $250 million Series C round.

February

• Together AI raised $305 million Series B, pushing its valuation to $3.3 billion.
• The AI hardware startup EnCharge AI also raised $100 million Series B.

January

• ElevenLabs raised $180 million in Series C to reach over $3 billion in valuation, while Hippocratic AI secured $141 million for healthcare-focused AI models.

As the year unfolds, 2025 appears poised to outperform 2024, maintaining the momentum of the AI funding landscape in the U.S.

For more details on specific funding rounds, check out the full list of U.S. AI startups that have raised $100 million or more in 2025 here.

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