55 US AI Startups That Secured $100M+ in Funding in 2025

The AI industry began 2025 on a strong note, building on the momentum established in 2024, where 49 startups in the U.S. secured funding rounds exceeding $100 million. Notably, three companies attracted multiple mega-rounds, and seven achieved funding of $1 billion or more.

In 2025, although fewer companies surpassed the $1 billion threshold, there was a remarkable increase in the number of firms securing multiple rounds of financing—eight in total, including Anthropic, which raised two rounds exceeding $1 billion. Significant early signs for 2026 emerged when Elon Musk’s xAI announced a staggering $20 billion Series E round, and Sam Altman’s Merge Labs raised a $250 million seed round led by OpenAI.

Here are some highlights from the significant funding activity in 2025:

December Highlights

• Mythic raised $125 million, enhancing their AI computing technology.
• Chai Discovery secured $130 million to further develop AI models for biotech.
• Fal gathered $140 million, tripling their valuation to over $4.5 billion.
• Unconventional AI raised a significant $475 million seed round.
• 7AI, specializing in AI for cybersecurity, raised $130 million.

November Highlights

• Genspark joined the unicorn club with a $275 million Series B round.
• Luma AI raised $900 million, positioning them at a $4 billion valuation.
• Anysphere saw a record-setting $2.3 billion funding round.

October Highlights

• Fireworks AI raised $250 million, while Uniphore collected $260 million in a Series F round.
• Sierra, a customer service AI platform, received $350 million, valuing the company at over $10 billion.

September Highlights

• Cognition AI raised $400 million, reaching a valuation of $10.2 billion.
• Anthropic raised a massive $13 billion Series F funding round.

This funding landscape demonstrates the astonishing trajectory of AI startups, as they adapt and innovate across various sectors, from healthcare to media. The pace and scale of investment signal a robust future for AI, with diverse applications that are increasingly becoming central to numerous industries.

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